Performing Enhanced Multiparameter Cell Imaging with Combined Fluorescence Lifetime Imaging Microscopy and Atomic Force Microscopy

optically encoded information about processes in live cells. Atomic force microscopy, on the other hand, provides nanometer-resolved surface topography and mechanical information, and has recently been expanded to nanometerresolved live cell mechanical property mapping. The integration of the two advanced live cell imaging techniques into one tool, with the capability to acquire simultaneous nanometer-mechanical and optical/biochemical information promises to provide new insights into cell mechanics. This application note describes the integration, demonstrated combined performance, and overlaid data of a cellular model system on the MicroTime 200 from PicoQuant and Bruker’s BioScope CatalystTM Atomic Force Microscope (AFM), on the basis of an IX71/81 Inverted Optical Microscope with PiFoc focus control.